PEPperCHIP® Peptide Microarrays are printed with influenza virus hemagglutinin (HA) epitope peptides as built-in positive controls. They are usually arranged as a single spot frame around the main peptide content for each array copy. When visualized with immunofluorescence, these peptide spots help in aligning the .gal grid during data quantification. For optimal visualization, we recommend the use of our PEPperCHIP® anti-HA control antibodies for control staining.
Product information
• | Mouse monoclonal anti-HA control antibody |
• | Fluorescent label: Cy5 for compatible microarray scanners (e.g., GenePix or Agilent) |
• | Volume: 10 µl at a stock concentration of 1 mg/ml |
• | Storage: 4oC or at -20oC for longer term |
Assay information
• | A working dilution of 1:2000 is recommended |
Estimated delivery time
• | 1-5 days after order confirmation |
• | 1-4 weeks when requested with PEPperCHIP® Peptide Micorarrays |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
<s>Take full control and perform PEPperCHIP® immunoassays at your own facility. Test multiple samples or multiple microarrays at once. Easy to assemble and fully reusable.</s>
<s>TBD.00 EUR</s>
<s>Excl. tax and shipping costs.</s>
<s>Request a Quote »</s>
