Protein/Peptide-to-Peptide Analysis
Identify and characterize protein or peptide interaction binding sites
PEPperCHIP® Peptide Microarrays are not only compatible with antibodies or sera, but also support analysis of proteins or peptides. Screen for protein ligands, characterize protein interactions by peptide mapping, or validate and optimize peptide leads (e.g. from display technologies) in high-throughput, analyzing up to 35,000 peptides in a single assay.
Screen your protein or peptide against any of our Standard Peptide Microarrays, or design your own peptide library. Depending on your sample characteristics, an additional assay optimization step may be added to your PEPperMAP® Protein+ Service.
PEPperCHIP® Discovery format | PEPperCHIP® Standard format | |
|---|---|---|
| Microarray content | Single to multiple antigens translated into overlapping peptides | Single to multiple antigens translated into overlapping peptides |
| Pre-designed content available | PEPperCHIP® Human Epitome Microarray, or random peptides for immunosignature discovery | PEPperCHIP® Standard Peptide Microarrays (over 40 different microarrays available) |
| Number of peptides | Up to 35,000 (in duplicate) | Up to 5,500 (in duplicate) |
| Available peptide conformations | Linear and cyclic constrained peptides | Linear and cyclic constrained peptide |
| Built-in control peptides | HA (YPYDVPDYAG) epitopes by default | HA (YPYDVPDYAG) epitopes by default |
| Input data (for custom content) | Peptide list, Database ID, or protein sequence(s) in the FASTA format | Peptide list, Database ID, or protein sequence(s) in the FASTA format |
| Sample material | At least 100 µg per sample | At least 25 µg per sample |