Protein/Peptide-to-Peptide Analysis
Identify and characterize protein or peptide interaction binding sites
PEPperCHIP® Peptide Microarrays are not only compatible with antibodies or sera, but also support analysis of proteins or peptides. Screen for protein ligands, characterize protein interactions by peptide mapping, or validate and optimize peptide leads (e.g. from display technologies) in high-throughput, analyzing up to 35,000 peptides in a single assay.
Screen your protein or peptide against any of our Standard Peptide Microarrays, or design your own peptide library. Depending on your sample characteristics, an additional assay optimization step may be added to your PEPperMAP® Protein+ Service.
PEPperCHIP® Discovery format | PEPperCHIP® Standard format | |
|---|---|---|
| Microarray content | Single to multiple antigens translated into overlapping peptides | Single to multiple antigens translated into overlapping peptides |
| Pre-designed content available | PEPperCHIP® Human Epitome Microarray, or random peptides for immunosignature discovery | PEPperCHIP® Standard Peptide Microarrays (over 40 different microarrays available) |
| Number of peptides | Up to 35,000 (in duplicate) | Up to 5,500 (in duplicate) |
| Available peptide conformations | Linear and cyclic constrained peptides | Linear and cyclic constrained peptide |
| Built-in control peptides | HA (YPYDVPDYAG) epitopes by default | HA (YPYDVPDYAG) epitopes by default |
| Input data (for custom content) | Peptide list, Database ID, or protein sequence(s) in the FASTA format | Peptide list, Database ID, or protein sequence(s) in the FASTA format |
| Sample material | At least 100 µg per sample | At least 25 µg per sample |
How to get started with your PEPperMAP® Service
Step 1
Tell us about your targets
If you already know which peptides or proteins you would like to screen, simply send us the sequence information via email, or through our request forms. This information will help us design a custom or select a pre-designed microarray for your project.
Unsure of your target? Let us know using the form below, and one of our specialists will get in touch to help design a chip for you.
Step 2
Select your service package
The comparison guide below outlines typical inclusions and available customizations for PEPperMAP® Protein+ Services. Prices and turnaround times may vary depending on the number of samples, complexity of experimental setup, and the type of data analysis requested.
To get a quote, simply fill in the request form at the end of the page.
Step 3
Send us your samples
Our microarrays can be used to analyze peptides, antibodies or nanobodies, sera, or even full proteins. The amount and concentration of samples you would need to send us will depend on the experimental setup, which will also be covered in the project consultation.
For custom microarray projects, chips will include positive assay controls compatible to your biological samples and experimental aim.
PEPperMAP® Essential covers the basic service componets necessary to deliver raw sample analysis data. The following are included:
PEPperCHIP® Custom or Standard Peptide Microarrays | |
• | Your protein or peptide sequence/s as overlapping peptides, or any of our pre-designed PEPperCHIP® Standard Peptide Microarrays |
• | Peptides printed in duplicate per array copy |
• | Built-in postive controls: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT), c-Myc (EQKLISEEDL) or custom peptides by request |
or, when selected,
HuProtTM Human Proteome Microarray v4.0 (available for customers within Europe) | |
• | Functional protein microarray |
• | Over 21,000 human proteins, isoform variants, and protein fragments |
Data Acquisition | |
• | All immunoassays with your samples or analytes |
• | Staining with control and secondary antibodies |
• | High-resolution scanning of processed micorarray and read-out data export |
Data Quantification and Formatting | |
• | Quantification of microarray data |
• | Data formatting in Excel |
• | Generation of data summaries and charts |
PEPperMAP® Standard includes everything int the Essential Package, along with some additional reporting features. It is our recommended cost-effective solution for projects with multiple samples or sample groups.
PEPperCHIP® Custom or Standard Peptide Microarrays | |
• | Your protein or peptide sequence/s as overlapping peptides, or any of our pre-designed PEPperCHIP® Standard Peptide Microarrays |
• | Peptides printed in duplicate per array copy |
• | Built-in postive controls: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT), c-Myc (EQKLISEEDL) or custom peptides by request |
or, when selected,
HuProtTM Human Proteome Microarray v4.0 (available for customers within Europe) | |
• | Functional protein microarray |
• | Over 21,000 human proteins, isoform variants, and protein fragments |
Data Acquisition | |
• | All immunoassays with your samples or analytes |
• | Staining with control and secondary antibodies |
• | High-resolution scanning of processed micorarray and read-out data export |
Data Quantification and Formatting | |
• | Quantification of microarray data |
• | Data formatting in Excel |
• | Generation of data summaries and charts |
• | Epitope annotations |
Data Reporting | |
• | Scientific reporting (includes materials and methods and experimental section) |
• | Data evaluation and discussion |
For projects with multiple samples or experimental conditions, additional services may be available based on applicability:
Bioinformatic analyses | |
• | Extensive statistical analyses including data normalization, data clustering or the generation of heat maps can be performed based on the scientific questions required by the project |
Affinity determination service | |
• | Kd/EC50 determination via fluorescence polarization assays |
Hit validation services | |
• | ELISA- or Luminex-based validation immunoassays |