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PEPperMAP® Services

Send us your sequence and samples and we'll take care of the rest

PEPperPRINT offers comprehensive epitope identification, antibody characterization, and antigen discovery and services allowing users to fully benefit from PEPperCHIP® Peptide Microarrays without any need for laboratory infrastructure. 

We offer complete end-to-end service covering everything from microarray design and production, immunoassays, to data quantification and evaluation.

Watch our short video to learn how it works. 

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Get the information you need from your samples

PEPperPRINT offers different project types and workflows depending on the nature of your sample and the detail level of information you would like to see in your sample analysis report. We offer a non-binding consultation free of charge to determine which workflow would best be suited to meet your scientific aim and budget. Browse our offers by sample type.


Antibodies

For analysis of mono- or polyclonal antibodies, nanobodies, or similar anitbody fragments, we offer epitope mapping, cross-ractivity analyses, substitution scans or antigen discovery.

Antibody services


Sera+

For analysis of patient or animal sera, or other types of biological samles (e.g. plasma, CSF, etc.) available services include multi-isotype mapping for antibody profiling or serum biomarker discovery.

Serum+ services


Proteins

For analysis of proteins and peptides, we are able to offer protein-peptide interaction analyses using peptide microarrays.
 

Protein services

How to get started with your PEPperMAP® Service

Step 1

Tell us about your targets

If you already know which peptides, proteins or antigens you would like to screen, simply send us the sequence information via email, or through our request forms. This information will help us design a custom or select a pre-designed microarray for your project.

Unsure of your target? We can also help with antigen discovery. Learn how.

Step 2

Select your service package

Your sample type will determine which PEPperMAP® Services and customization options are available. Prices and turnaround times may vary depending on the number of samples, complexity of experimental setup, and the type of data analysis requested.

A comparison guide below outlines typical inclusions for each service package. Browse services by sample type for more details.

Step 3

Send us your samples

Our microarrays can be used to analyze peptides, antibodies or nanobodies, sera, or even full proteins. The amount and concentration of samples you would need to send us will depend on the experimental setup, which will also be covered in the project consultation.

For custom microarray projects, chips will include positive assay controls compatible to your biological samples and experimental aim.

PEPperMAP® Essential covers the basic service componets necessary to deliver raw sample analysis data. The following are included:

PEPperCHIP® Custom or Standard Peptide Microarrays

• 

Your protein or peptide sequence/s as overlapping peptides, or any of our pre-designed PEPperCHIP® Standard Peptide Microarrays

• 

Peptides printed in duplicate per array copy

• 

Built-in postive controls: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT), c-Myc (EQKLISEEDL) or custom peptides by request

  

or, when selected,

HuProtTM Human Proteome Microarray v4.0 (available for customers within Europe)

• 

Functional protein microarray

• 

Over 21,000 human proteins, isoform variants, and protein fragments

  

Data Acquisition

• 

All immunoassays with your samples or analytes

• 

Staining with control and secondary antibodies

• 

High-resolution scanning of processed micorarray and read-out data export

  

Data Quantification and Formatting

• 

Quantification of microarray data

• 

Data formatting in Excel

• 

Generation of data summaries and charts

  

PEPperMAP® Standard includes everything int the Essential Package, along with some additional reporting features. It is our recommended cost-effective solution for projects with multiple samples or sample groups.

PEPperCHIP® Custom or Standard Peptide Microarrays

• 

Your protein or peptide sequence/s as overlapping peptides, or any of our pre-designed PEPperCHIP® Standard Peptide Microarrays

• 

Peptides printed in duplicate per array copy

• 

Built-in postive controls: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT), c-Myc (EQKLISEEDL) or custom peptides by request

  

or, when selected,

HuProtTM Human Proteome Microarray v4.0 (available for customers within Europe)

• 

Functional protein microarray

• 

Over 21,000 human proteins, isoform variants, and protein fragments

  

Data Acquisition

• 

All immunoassays with your samples or analytes

• 

Staining with control and secondary antibodies

• 

High-resolution scanning of processed micorarray and read-out data export

  

Data Quantification and Formatting

• 

Quantification of microarray data

• 

Data formatting in Excel

• 

Generation of data summaries and charts

• 

Epitope annotations

  

Data Reporting

• 

Scientific reporting (includes materials and methods and experimental section) 

• 

Data evaluation and discussion

  

For projects with multiple samples or experimental conditions, additional services may be available based on applicability:  

Bioinformatic analyses

• 

Extensive statistical analyses including data normalization, data clustering or the generation of heat maps can be performed based on the scientific questions required by the project

  

Binding motif analyses

• 

Multiple Em for Motif Elicitation (MEME) analyisis - discovery of common motifs, essential and conserved amino acid positions in the top peptide hits.

• 

Find Individual Motif Occurences (FIMO) analysis - database blasting of MEME selected motifs to identify protein candidates for cross-reactions.

  

Affinity determination service

• 

Kd/EC50 determination via fluorescence polarization assays

  

Hit validation services

• 

ELISA- or Luminex-based validation immunoassays 

  

 

 

Frequently Asked Questions

A PEPperMAP® Service typically includes all immunoassays and related materials (ie., PEPperCHIP® Peptide Microarrays, HuProtTM Protein Microarrays, Luminex MAGPIX® etc. as defined by the project), as well as data acquisition perfomed at our service laboratory. Depending on the nature of your samples and the level of detail requested in the sample analysis report, the PEPperMAP® Service may also inlcude:

  • Formatted raw data and charts as xlsx files
  • Epitope annotations
  • Written materials and methods
  • Microarray scans
  • Bioinformatic analyses
  • Detailed discussion and interpretation of the data
 

    PEPperMAP® Service costs are calculated based on the time and material needed for thorough service. Standard PEPperMAP® Epitope Mapping Services with an antibody or serum sample against a single protein are covered by flat-rate fees that are widely independent of the length of the protein sequence. Please do not hesitate to contact us for more information and nonbinding cost estimations for the intended research project. We guarantee to always provide you with the most cost effective PEPperMAP® Service solutions available.

    The timeline starts with receipt of the antigen sequence, followed by 4 weeks for peptide microarray generation. We usually ask to ship the sample(s) before peptide microarray finalization. Subsequently, the assays will take around 3 weeks followed by another week for data evaluation and quantification. In total, it takes around 8 weeks for the full process until delivery of the report.

    For service to start, we need a purchase order containing the order number and the billing address. Furthermore, we need the peptide list/antigen sequence (FASTA format or Uniprot ID). We will then send you an order confirmation and the layout of the microarray (peptide map) for your approval. After your approval, we will start with the peptide synthesis which will take about 3-4 weeks. During this time, you can send the samples to us. Please inform us as soon as you send the samples and complete the PEPperMAP® Sample Submission Form (containing information about the sample name, storage temperature etc.). Payment can be done via bank transfer (after receiving the invoice) or by credit card.

    PEPperPRINT GmbH
    Tullastr. 2
    69126 Heidelberg
    Germany

    Please complete and return the PEPperMAP® Sample Submission Form (available in our downloads section) along with your antibody, serum, or protein sample(s) to ensure proper handling and storage.

    Most customers send us antibody or serum samples on ice packs using FedEx. If you would like to use dry ice, fill the box half with dry ice. Seal your tubes with parafilm, put them in a plastic bag and put this bag directly in the dry ice. Fill the empty space with Styrofoam to prevent that the dry ice is shaken too much.

    Please describe your goods precisely on the Air Waybill (e.g. human monoclonal antibody, murine serum, synthetic antibody). If you use e.g. “reagent for research” or “protein solution”, FedEx will ask us for more information, which can delay sample shipment.

    For analysis of animal-derived antibodies and sera that are shipped from overseas (e.g. from USA), please complete and return the PEPperMAP® Sample Declaration Form along with your samples to avoid any clearance delay. Animal derived samples are randomly checked by the veterinary office at the airport in Cologne. The veterinary office wants to make sure that no animal diseases or pathogenic agents are introduced into Germany. An inspection by the veterinary office may lead to extra costs.

    To import animal derived samples into Germany, it is necessary that you state in the PEPperMAP® Sample Declaration Form (available in our downloads section) that your samples have been separated from plasma and are highly purified, so that animal disease or other pathogenic agents were killed off or effectively removed. Please insert the sample nature and state how the samples were produced. Please attach this form to your shipment documents to the outside of the box.

    If you have animal serum samples that are not purified, please contact us for further details. Human serum samples and synthetic proteins or antibodies do not require the aforementioned  form.

    First, we need the antigen sequence in FASTA format or the Uniprot ID. We will discuss with you how much sample we need to run the assay services for your specific project.

    The amount of sample needed depends on different factors. One is the incubation volume that depends on the size of the array. Moreover, it depends on the type of sample used. Last but not least, we test up to three different dilutions for incubation in order to obtain the best results possible. The exact amount of sample needed can therefore only be determined individually for each project.

    Please send us your protein sample/antibody without BSA and without sodium azide. Both additives reduce the labeling efficiency.

    Ready to discuss your project?

    Talk to one of our specialists to get started

    Get in touch »Request a quote »

    Did you know you can also perform PEPperCHIP® experiments at your own lab?

    Explore PEPperCHIP® Peptide Microarrays and Accessories to learn more