We highly recommend using Rockland blocking buffer (MB-070) for your PEPperCHIP® Peptide Microarray experiments. It blocks unspecific protein binding on the microarray, thereby effectively reducing background signals to generate high-quality data. Rockland blocking buffer is used for all PEPperMAP® Services.
Product information
• | Compatibility: can be used for blocking either linear or conformational PEPperCHIP® Peptide Micorarrays |
• | Volume: 500 mL (sterile filtered) |
• | Dilution: Optimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance |
Storage information
• | Store at 4oC prior to opening |
| Do not freeze |
Estimated delivery time
• | 1-5 days after order confirmation |
• | 1-4 weeks when requested with PEPperCHIP® Peptide Micorarrays |
Documentation
• | Rockland Blocking Buffer Datasheet [download pdf] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Take full control and perform PEPperCHIP® immunoassays at your own facility. Test multiple samples or multiple microarrays at once. Easy to assemble and fully reusable.
TBD.00 EUR
Excl. tax and shipping costs.
