The PEPperCHIP® Chikungunya Virus Microarray covers the entire Chikungunya viral proteome translated into highly overlapping peptides. The microarray chip contains 3,728 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
Identify, profile and compare Chikungunya virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Chikungunya related antigens (strain S27 - African prototype): nonstructural protein BHI3734/H804698 ESCA (ID: Q8JUX6), structural polyprotein BHI3741/H804705 ESCA (ID: Q8JUX5): Proteomes of the abovementioned strain as available on UniProt-or NCBI-database |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 3,728 / 7,456 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 44 and 32 and spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Chikungunya Virus Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Chikungunya Virus Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Analysis of Humoral Immune Responses in Chikungunya Virus (CHIKV) Infected Patients and Individuals Vaccinated with a Candidate CHIKV Vaccine.
Lisa Henss, Constanze Yue, Christine von Rhein, Roland Tschismarov, Lia Laura Lewis-Ximenez, Albert Dölle, Sally A Baylis, and Barbara S Schnierle. The Journal of Infectious Diseases, December 12, 2019, jiz658. [doi.org/10.1093/infdis/jiz658]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
