The PEPperCHIP® Cyclic Citrullinated Peptide Microarray covers 577 different peptides printed in duplicate. The microarray chip contains 337 different cyclic citrullinated peptides and the corresponding arginine variants. Peptide cyclization has been achieved by thioether cyclization between C-terminal cysteine and modified N-terminus. The microarray chip contains five array copies for one-by-one assays including HA, polio and c-Myc positive control peptides.
The microarray can be used epitope mapping, anti-CCP serum screening or biomarker discovery of autoantigens.
Microarray information
• | Microarray content: 337 cyclic citrullinated peptides of the Immune Epitope Database and from literature including the corresponding arginine variants. |
• | Peptide length and overlap: 10 aa - 17 aa cyclic constrained peptides via N- to C-terminal thioether formation |
• | Number of peptides / spots: 577 / 1,154 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (26, 22 and 22 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 5 array copies per chip |
• | Accessories: compatible with 3/5 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Cyclic Citrullinated Peptide Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Cyclic Citrullinated Peptide Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
