The PEPperCHIP® Epstein-Barr Virus Peptide Microarray includes 18 of the most immunogenic Epstein-Barr Virus antigens converted into 5,549 highly overlapping peptides. The microarray chip contains linear 13-mer peptides with a 11-mer overlap (shift 2) in duplicates for full epitope coverage. Moreover, the microarray contains HA and polio positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: 18 of the most immunogenic antigens of Epstein-Barr virus |
• | Peptide length and overlap: 13 aa peptides with peptide-peptide overlap of 11 aa (shift 2) |
• | Number of peptides / spots: 5,549 / 11,098 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) control peptides (26 and 21 spots each control, respectively) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Epstein-Barr Virus Peptide Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Epstein-Barr Virus Peptide Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Molecular Mimicry, Genetic Homology, and Gene Sharing Proteomic ‘Molecular Fingerprints’ Using an EBV (Epstein-Barr Virus)-Derived Microarray as a Potential Diagnostic Method in Autoimmune Disease.
David H. Dreyfus, Antonella Farina, and Giuseppina Alessandra Farina. Immunologic Research 66, no. 6 (December 2018): 686–95. [doi.org/10.1007/s12026-018-9045-0]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
