The PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray covers the entire foot-and-mouth disease virus proteome translated into highly overlapping peptides. The microarray chip contains 2,332 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
Identify, profile and compare Foot-and-mouth Disease Virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Genome polyprotein of foot-and-mouth disease virus cleaved into 15 chains: Leader protease (Lpro), protein VP0 (VP4-VP2), protein VP4 (P1A, Virion protein 4), protein VP2 (P1B, Virion protein 2), protein VP3 (P1C, Virion protein 3), protein VP1 (P1D, Virion protein 1), protein 2A (P52), Protein 2B, protein 2C, protein 3A, protein 3B-1 (Genome-linked protein VPg1), protein 3B-2 (Genome-linked protein VPg2), protein 3B-3 (Genome-linked protein VPg3), picornain 3C (Protease 3C, Protease P20B), RNA-directed RNA polymerase 3D-POL (P56A) |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 2,332 / 4,664 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 36 and 26 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Universal Detection of Foot and Mouth Disease Virus Based on the Conserved VP0 Protein.
Silvia Loureiro, Claudine Porta, Hemanta K. Maity, Eva Perez, Flavia F. Bagno, Abhay Kotecha, Elizabeth Fry, et al. Wellcome Open Research 3 (July 25, 2018): 88. [doi.org/10.12688/wellcomeopenres.14655.1]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
