The PEPperCHIP® HBV Proteome Microarray covers the entire HBV proteome translated into overlapping peptides. The microarray chip contains 4,731 linear 15-mer peptides with a 12-mer overlap (shift 3) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
Identify, profile and compare HBV specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Proteomes of all different genotypes, and various subtypes, of HBV; linked and elongated with neutral GSGSGSG linkers at the C- and N-terminus and translated into overlapping peptides. Redundant peptides were removed. |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 12 aa (shift 3) |
• | Number of peptides / spots: 4,713 / 9,426 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 36 and 24 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® HBV Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® HBV Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Predicting HBsAg Clearance in Genotype A Chronic Hepatitis B Using HBsAg Epitope Profiling: A Biomarker for Functional Cure.
Renae Walsh, Rachel Hammond, Lilly Yuen, Joshua Deerain, Tanya O’Donnell, Thomas Leary, Gavin Cloherty, et al. Liver International 39, no. 11 (November 2019): 2066–76. [doi.org/10.1111/liv.14207]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
