The PEPperCHIP® HCMV Antigen Microarray covers 24 selected antigens from human cytomegalovirus translated into 4,327 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 12-mer overlap (shift 3) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: 24 selected antigens of human cytomegalovirus as overlapping linear peptides |
• | Peptide length and overlap: 15 aa peptides with a peptide-peptide overlap of 12 aa (shift 3) |
• | Number of peptides / spots: 4,327 / 8,654 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® HCMV Antigen Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® HCMV Antigen Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Impact of Antibodies and Strain Polymorphisms on Cytomegalovirus Entry and Spread in Fibroblasts and Epithelial Cells.
Xiaohong Cui, Daniel C. Freed, Dai Wang, Ping Qiu, Fengsheng Li, Tong-Ming Fu, Lawrence M. Kauvar, and Michael A. McVoy. Edited by Klaus Frueh. Journal of Virology 91, no. 13 (July 1, 2017): e01650-16, /jvi/91/13/e01650-16.atom. [doi.org/10.1128/JVI.01650-16]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
