The PEPperCHIP® HIV-1 (Env) Antigen Microarray covers HIV-1 envelope protein gp160 (gp120 + gp41) translated into 856 highly overlapping linear peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Each microarray chip contains three array copies for one-by-one assays. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Envelope glycoprotein (Env) gp160 of HIV type 1 cleaved into 2 chains: Surface protein gp120 and Transmembrane protein gp41 |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 856 / 1,712 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (22, 28 and 20 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/3 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® HIV-1 (Env) Antigen Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® HIV-1 (Env) Antigen Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
HIV-1 Gp140 Epitope Recognition Is Influenced by Immunoglobulin DH Gene Segment Sequence.
Yuge Wang, Pratibha Kapoor, Robert Parks, Aaron Silva-Sanchez, S. Munir Alam, Laurent Verkoczy, Hua-Xin Liao, et al. Immunogenetics 68, no. 2 (February 2016): 145–55. [doi.org/10.1007/s00251-015-0890-x]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
