The PEPperCHIP® Mpox Virus Peptide Microarray includes 33 proteins of the Mpox virus (strain Zaire-96-I-16) converted into 5,207 different overlapping peptides. The microarray chip contains linear 15 amino acid peptides with a 13 amino acid overlap (shift 2) printed in duplicates for full epitope coverage. Moreover, the microarray contains additional HA and polio positive control peptides.
The microarray can be used for the antibody fingerprint analysis from serum or plasma for antibody biomarker discovery or vaccine development, but also for the multiplexed epitope mapping of antibodies against Mpox virus
Microarray information
• | Microarray content: 33 proteins of the Mpox virus (strain Zaire-96-I-16) (MPX) |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2) |
• | Number of peptides / spots: 5,207 / 10,414 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) control peptides (114 and 112 spots each control, respectively) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Mpox Virus Peptide Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Mpox Virus Peptide Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
