Profile antibody responses against the most important wild type AAV capsid proteins with the PEPperCHIP® Pan-AAV Capsid Protein Microarray. The chip contains the sequences of 15 different capsid proteins converted into more than 5,000 overlapping peptides for high-resolution epitope data. Available with linear or cyclic constrained peptides for linear or conformational epitope mapping, the PEPperCHIP® Pan-AAV Capsid Protein Microarray can be used to analyze patient and animal sera as well as research and diagnostic anti-AAV antibodies on the epitope level.
The PEPperCHIP® Pan-AAV Capsid Protein Microarray can be used for the following applications
• | Anti-AAV antibody monitoring in patient sera before and after gene therapy |
• | Identification of cross-reacting anti-AAV antibodes |
• | Identification of pre-existing anti-AAV antibody responses in healthy individuals |
• | Fingerprint analysis of anti-AAV antibody responses in animal models |
• | Validation of research or diagnostic antibodies against AAVs |
Microarray information
• | Microarray content: Capsid proteins of AAV1 (protein ID NP_049542.1), AAV2 (protein ID P03135), AAV3b (protein ID O56139), AAV4 (protein ID O41855), AAV5 (protein ID Q9YIJ1), AAV6 (protein ID O56137), AAV7 (protein ID Q8JQG0), AAV8 (protein ID Q8JQF8), AAV9 (protein ID AAS99264.1), AAVrh.10 (protein ID AAO88201.1), AAVpo.1 (protein ID ACN42940.1), AAV10 (protein ID AAT46337.1), AAV11 (protein ID AAT46339.1), AAV12 (protein ID ABI16639.1) and AAV13 (protein ID ABZ10812) |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2) |
• | Number of peptides / spots: Conformational microarray - 5,537 / 11,068 (peptides printed in duplicate); Linear microarray - 5,519 / 11,038 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) control peptides |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Conformational Pan-AAV Capsid Protein Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Conformational Pan-AAV Capsid Protein Microarray Peptide Map [download xlsx] |
• | PEPperCHIP® Linear Pan-AAV Capsid Protein Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Linear Pan-AAV Capsid Protein Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
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Identify, profile, and compare AAV-specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development, or screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research.
475.00 EUR
Excl. tax and shipping costs.
