The PEPperCHIP® Signature Discovery Microarray covers 29,240 randomized 15-mer linear peptides with varying amino acid distribution in duplicate. Moreover, the microarray contains HA, polio and c-Myc positive control peptides. The microarray can be used for epitope mapping, serum screening, antigen discovery as well as antibody cross-reactivity analysis.
Please note that this peptide microarray is only available in combination with our PEPperMAP® Services.
Microarray information
• | Microarray content: 29,240 randomized peptides with the following amino acid frequencies: 6% for R, S, T, W, Y; 5% for A, D, E, F, G, H, I, K, L, M, N, P, Q, V |
• | Peptide length: 15 aa |
• | Number of Peptides / Spots: 29,240 / 58,480 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (261, 257 and 257 spots each control) |
• | Size and format: Discovery Format (10.5 cm x 11 cm) containing 1 array copy per chip |
• | Accessories: only available in combination with our PEPperMAP® Services |
Sample information
• | Can be assayed with at least 100 µL serum/plasma or 100 µg/mL purified antibody. |
Estimated delivery time
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Signature Discovery Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Signature Discovery Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
High-Throughput Epitope Profiling of Antibodies in the Plasma of Alzheimer’s Disease Patients Using Random Peptide Microarrays.
Kyu-Young Sim, Sang-Heon Park, Kyu Yeong Choi, Jung Eun Park, Jung Sup Lee, Byeong C. Kim, Jeonghwan Gwak, Woo Keun Song, Kun Ho Lee, and Sung-Gyoo Park. Scientific Reports 9, no. 1 (December 2019): 4587. [doi.org/10.1038/s41598-019-40976-x]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
