The PEPperCHIP® Tetanus Toxin Microarray covers the whole Tetanus toxin antigen translated into 1,315 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Each microarray chip contains three two array copies for one-by-one assays. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Tetanus toxin (Tentoxylysin) from Clostridium tetani (strain Massachusetts / E88) |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 1,315 / 2,630 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (31, 30 and 22 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 2 array copies per chip |
• | Accessories: compatible with 3/2 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Tetanus Toxin Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Tetanus Toxin Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
An Oligoclonal Combination of Human Monoclonal Antibodies Able to Neutralize Tetanus Toxin in Vivo.
Eduardo Aliprandini, Daniela Yumi Takata, Ana Lepique, Jorge Kalil, Silvia Beatriz Boscardin, and Ana Maria Moro. Toxicon: X 2 (April 2019): 100006. [doi.org/10.1016/j.toxcx.2019.100006]
Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution.
Andrea Palermo, Laura K. Weber, Simone Rentschler, Awale Isse, Martyna Sedlmayr, Karin Herbster, Volker List, et al. Biotechnology Journal 12, no. 10 (October 2017): 1700197. [doi.org/10.1002/biot.201700197]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
