The PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray covers the entire Tick-borne Encephalitis Virus translated into highly overlapping peptides. The microarray chip contains 3,414 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
Identify, profile and compare Tick-borne Encephalitis Virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Genome polyprotein of tick-borne encephalitis virus cleaved into 13 chains: peptide 2k, capsid protein C (core protein), prM, peptide pr, small envelope protein M (matrix protein), envelope protein E, non-structural protein 1, non-structural protein 2A, serine protease subunit NS2B (flavivirin protease NS2B regulatory subunit, non-structural protein 2B), serine protease NS3 (flavivirin protease NS3 catalytic subunit, non-structural protein 3), non-structural protein 4A, non-structural protein 4B, RNA-directed RNA polymerase NS5 (non-structural protein 5) |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 3,414 / 6,828 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (37, 32 and 24 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Antibody Fingerprints in Lyme Disease Deciphered with High Density Peptide Arrays.
Laura K. Weber, Awale Isse, Simone Rentschler, Richard E. Kneusel, Andrea Palermo, Jürgen Hubbuch, Alexander Nesterov-Mueller, Frank Breitling, and Felix F. Loeffler. Engineering in Life Sciences 17, no. 10 (October 2017): 1078–87. [doi.org/10.1002/elsc.201700062]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
