The PEPperCHIP® Tumor Antigen Microarray covers 22 selected onco-antigens translated into 4,319 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 13-mer overlap (shift 2) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: histone H1.2, histone H4, G2/mitotic-specific cyclin-B1, L-dopachrome tautomerase, cellular tumor antigen p53, receptor tyrosine-protein kinase erbB-2, mammaglobin A, TCRgamma alternate reading frame protein, stromelysin-3 (MMP11), protein SSX2 (Cancer/testis antigen 5.2), prostate-specific antigen, cancer/testis antigen 1 (NY-ESO-1), Myc proto-oncogene protein, claudin-6, carcinoembryonic antigen-related cell adhesion molecule 5, Melanocyte protein PMEL, Wilms tumor protein, tyrosinase, transcription factor SOX-2, myelin-oligodendrocyte glycoprotein, actin, alpha skeletal muscle, HLA class I histocompatibility antigen, A-36 alpha chain |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2) |
• | Number of peptides / spots: 4,319 / 8,638 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 28 and 24 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Tumor Antigen Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Tumor Antigen Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
