The PEPperCHIP® West Nile Virus Proteome Microarray covers the viral genome polyprotein converted into highly overlapping peptides. The microarray chip contains 3,430 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
Identify, profile and compare West Nile Virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Genome polyprotein of West Nile virus cleaved into 13 chains: peptide 2k, capsid protein C, prM, peptide pr, small envelope protein M, envelope protein E, non-structural protein 1, non-structural protein 2A, serine protease subunit NS2B, serine protease NS3, non-structural protein 4A, non-structural protein 4B, RNA-directed RNA polymerase NS |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 3,430 / 6,860 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 32, 22 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® West Nile Virus Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® West Nile Virus Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
