The PEPperCHIP® Zika Virus Proteome Microarray covers the entire Zika Virus from two different strains translated into highly overlapping peptides. The microarray chip contains 3,419 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
Identify, profile and compare Zika Virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Zika virus genome polyprotein cleaved into 13 chains: protein C, protein prM, peptide pr, small envelope protein M, envelope protein E, non-structural protein 1, non-structural protein 2A, serine protease subunit NS2B, serine protease subunit NS3, non-structural protein 4A, peptide 2K, non-structural protein 4B, RNA-directed RNA polymerase NS5 |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 3,419 / 6,838 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (34, 32 and 26 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Zika Virus Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Zika Virus Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Identification of a Zika NS2B Epitope for Which Absence of IgG Response Is Associated with Severe Neurological Symptoms and the Design of a Biomarker Capable of Discriminatory Diagnostics Between Severe and Non Severe Clinical Phenotypes.
Felix Loeffler, Isabelle F. T. Viana, Nico Fischer, Danilo F. Coêlho, Carolina Santos, Antonio Purificacao Jr, Catarina Araujo, et al., 8. March 2021. [doi.org/10.26434/chemrxiv.14170439.v1]
Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses.
Marjorie C. L. C. Freire, Laércio Pol-Fachin, Danilo F. Coêlho, Isabelle F. T. Viana, Tereza Magalhães, Marli T. Cordeiro, Nico Fischer, et al. ACS Omega 2, no. 7 (July 31, 2017): 3913–20. [doi.org/10.1021/acsomega.7b00608]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
