The PEPperCHIP® Herpes-Simplex-Virus 1 Proteome Microarray covers all 74 HSV-1 proteins including all envelope glycoproteins converted into 19,408 different 15 amino acid peptides with a peptide overlap of 13 amino acids for high resolution epitope data. In addition, the microarray contains HA and polio positive control peptides.
Identify, profile and compare HSV-1 specific antibody responses on the epitope level for antibody biomarker discovery, antibody validation or vaccine development. Identify immunodominant anti-HSV-1 antibody responses or epitope signatures with a diagnostic or prognostic potential. Correlate antibody profiles with a potential role of HSV-1 in dementia or Alzheimer's disease. The microarray can be used for proteome-wide epitope mapping of anti-HSV-1 antibody responses, serum antibody profiling or biomarker discovery.
Microarray information
• | Microarray content: 74 HSV-1 proteins including all envelope glycoproteins were converted into overlapping peptides |
• | Peptide length and overlap: 15 aa peptides with an overlap of 13 aa (shift 2) |
• | Number of peptides / spots: 19,4084 / 38,816 (peptides printed in duplicate) |
• | Positive controls: HA (481 spots, YPYDVPDYAG) and polio (436 spots, KEVPALTAVETGAT) control peptides |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Herpes-Simplex-Virus 1 Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Herpes-Simplex-Virus 1 Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
